Humoral immunity evaluation and virual challenge of lysosomal targeted hantavirus envelope glycoprotein DNA vaccine
10.3969/j.issn.1000-484X.2018.03.003
- VernacularTitle:溶酶体靶向的汉坦病毒包膜糖蛋白DNA疫苗的体液免疫评价和攻毒保护效果
- Author:
Jin-Peng ZHANG
1
;
Guan-Wen ZHANG
;
Guo-Yun XUAN
;
Huan ZHANG
;
Dong-Bo JIANG
;
Kun YANG
Author Information
1. 第四军医大学基础部免疫学教研室
- Keywords:
Hantavirus envelope glycoprotein;
DNA vaccine;
LAMP;
Immunological evaluation
- From:
Chinese Journal of Immunology
2018;34(3):331-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To build the DNA vaccine encoding hantavirus glycoprotein fused with lysosome-associated membrane protein (LAMP) and assess its immune response.Methods:BALB/c mice were immuned with the previous experimental expressing purified recombinant plasmids pVAX-Gn,pVAX-Gc,pVAX-LAMP/Gn,pVAX-LAMP/Gc,and inactivated vaccine.Indirect ELISA and neutralization test was used to detect specific antibody and neutralizing antibody in the serum of mice.The mice were tested to detect the protective effects in vivo.Results:Indirect ELISA results showed that the pVAX-LAMP/Gc group was the highest,followed by pVAX-Gc,pVAX-LAMP/Gn,and PVAX-Gn,and inactivated vaccine group.In neutralization test,there were significantly higher serum antibodies in LAMP group than those in conventional DNA group,which were higher than the inactivated vaccine group.The mice immuned had good protective effect in vivo without the specific antigen of hantavirus in vivo.Conclusion:Chimeric DNA vaccines induced higher levels of antibody in BALB/c mice and produced better protective efficacy,which illustrate the targeting strategy is expected to be an effective way to improve the DNA vaccine efficacy.
