Effects of Ding Kundan on expression of TGF-β1,CTGF and AR in PCOS model rats
10.3969/j.issn.1000-484X.2018.02.012
- VernacularTitle:定坤丹对PCOS模型大鼠TGF-β1、CTGF和AR表达的影响
- Author:
Lan CHEN
1
;
Yong TAN
;
Shu-Ping CHEN
Author Information
1. 南京中医药大学
- Keywords:
Ding Kundan;
Polycystic ovary syndrome;
Transforming growth factor-beta 1;
Connective tissue growth factor;
Androgen receptor
- From:
Chinese Journal of Immunology
2018;34(2):218-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Ding Kundan on the expression of TGF-β1,CTGF and AR in PCOS model rats,and to explore its treat mechanism on PCOS.Methods: The PCOS model rats induced by DHEA were intervened with high, medium and low dose of Ding Kundan(4.5,2.25,1.125 g/kg),ELISA,immunohistochemistry,RT-PCR and Western blot were used to detect serum hormone levels,mRNA and protein expression of TGF-β1,CTGF and AR in each group of rats.Results: Compared with model group Ding Kundan high,medium and low dose group of E2,LH and T were decreased,while FSH was increased.And the differences were statistically significant.Protein expression and mRNA of TGF-β1,CTGF and AR were decreased,and the differences were statistically significant.Conclusion: Ding Kundan shows significantly therapeutic efficacy on PCOS rats,and its possible mechanism is related to regulating expression levels of TGF-β1,CTGF and AR in ovarian tissue.
