Effect of Lycium Barbarum polysaccharides on immune function of erythrocytes in immunosuppressed mice
10.3969/j.issn.1000-484X.2018.02.011
- VernacularTitle:枸杞多糖对免疫抑制小鼠红细胞免疫功能的影响
- Author:
Zhen-Hua HUANG
1
;
Xiang-Liang DENG
;
Kai-Min ZHANG
;
Liu-Ting LAI
;
Ming-Hua HU
;
Fang-Li MA
;
Xia LUO
;
Lian ZHOU
Author Information
1. 广州中医药大学中药学院中医药免疫研究室
- Keywords:
Lycium Barbarum polysaccharides;
Erythrocytes;
NKEF-A;
NKEF-B
- From:
Chinese Journal of Immunology
2018;34(2):214-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Lycium Barbarum polysaccharides on immune function of erythrocytes in doxorubicin-treated mice.Methods:BALB/c mice were treated with doxorubicin and used as immunosuppression model.The mice were treated with Lycium Barbarum polysaccharides(62.5,125,250 mg/kg) for 7 days,the normal control mice and model control mice were also used in this study.Expression level of CD59 molecule in erythrocytes was analyzed with flow cytometry.The Band-3 level was analyzed by Coomassie Brilliant Blue method.The NKEF-A and NKEF-B expression level in erythrocytes was analyzed by Western blot.The killing activity of NK cells was analyzed with flow cytometry.Results: The level of Band-3,NKEF-A and NKEF-B was decrease in erythrocytes of doxorubicin-treated mice.The killing activity of NK cells was also decrease in the mice when the expression level of CD59 molecule was not change obviously.Lycium Barbarum polysaccharides treatment could promote the recovery of Band-3, NKEF-A and NKEF-B in erythrocytes of the mice.Conclusion:Lycium Barbarum polysaccharides can promote the recovery of immune function of erythrocytes in doxorubicin-treated mice.
