Effects of downregulation of HIF-2α gene on biological behaviors of hepatoma cells induced by hypoxia in vitro
10.3969/j.issn.1000-484X.2018.02.008
- VernacularTitle:下调HIF-2α基因对低氧诱导的肝癌细胞体外生物学行为的影响
- Author:
Wei-Wei LI
1
;
Xiao-Song GENG
;
Bao-Sheng SHEN
;
Yu-Xin YANG
;
Xin-Wen SONG
Author Information
1. 新乡医学院第一附属医院感染疾病科
- Keywords:
Human hepatocellular carcinoma;
Hypoxia induced factors 2α;
RNA interference;
Invasion;
Cell apoptosis
- From:
Chinese Journal of Immunology
2018;34(2):199-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of hypoxia-inducible factor 2α genes on under hypoxia on proliferation,apoptosis, cell cycle distribution and migration of invasiveness of human hepatocellular carcinoma cell HepG2.Methods: Human hepatoma cell line HepG2 induced by cobalt chloride (CoCl2) was selected as the research object,construction of siRNA specific carrier HIF-2α, transfection of HepG2 cells under hypoxia.Real-time PCR,Western blot method in the detection of before and after transfection in each group of HIF-2α mRNA and protein expression;MTT method to detect the proliferation of HepG2 cells before and after transfection;apoptosis rate and distribution of cell cycle of HepG2 cells before and after transfection were detected by flow cytometry;Transwell test was used to detect the invasion and migration ability of HepG2 cells before and after transfection.Results: Under hypoxia,significant increased HIF-2α expression in hepatocellular carcinoma HepG2 cells.Specific transfection of HIF-2α siRNA in HepG2 cells after HIF-2α mRNA and protein expression levels were significantly inhibit cell proliferation decreased,apoptosis rate increased in the ratio of G0/G1 phase cells increased synthesis phase (S) and late (G2/M) synthesis cell ratio decreased,which in vitro invasion and migration of cells was inhibited.Conclusion:Expression of HIF-2α increases in hepatocellular carcinoma HepG2 cells under hypoxia. Specific siRNA can be cut by HIF-2α gene expression in HepG2 cells under hypoxia,to inhibit HepG2 cell proliferation,invasion, migration,and change the distribution of cell cycle and induce its apoptosis.
