Sesamin suppresses mast cell activation through inhibition of PKCα/NF-κB signaling pathway
10.3969/j.issn.1000-484X.2018.02.002
- VernacularTitle:芝麻素通过PKCα/NF-κB信号途径抑制肥大细胞活化
- Author:
Hong-Wei ZHAO
1
;
Yin-Feng CUI
;
Jing-Zhi JIANG
;
Nan CHE
;
Jing YE
;
Chong-Yang WANG
;
Liang-Chang LI
Author Information
1. 延边大学医学院
- Keywords:
Sesamin;
Mast cell;
Allergic reaction;
Cytokine;
NF-κB
- From:
Chinese Journal of Immunology
2018;34(2):167-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of sesamin on mast cell activation and its inflammatory mediator release,as well as its possible mechanisms of action.Methods:HCM-1 cells were activation by stimulation with 10 μg/ml anti-DNP IgE for 6 h and challenge with 100 ng/ml DNP-HAS for 10 min.Sesamin was administration at the concentration of 25,50 and 100 μg/L prior to DNP-HAS challenge,subsequently the effect of sesamin on mast cell degranulation was investigated by light microscope,and histamine release and expression of cytokines such as TNF-α IL-6,IL-1β,IL-8 of mast cells after sesamin treatment were investigated by ELISA.Western blot was used to determine the effect of sesamin on FcεRI downstream signaling including Lyn,Syk and PKCα activation,and IκBα phosphorylation and NF-κB activation.Results:DNP-HAS significantly increased mast cell degranulation,histamine release and those cytokines expression,enhanced Lyn,Syk,PKCα,IκBα phosphorylation and NF-κB activation(P<0.05). Sesamin(50,100 μg/L) significantly decreased mast cell degranulation,histamine release and cytokines expression (TNF-α,IL-4,IL-1β,and IL-8),reduced activity of Lyn,Syk,kinases and PKCα and IκBα phosphorylation,and inhibited NF-κB activation(P<0.05).Conclusion: Sesamin suppresses mast cell activation and inflammatory mediators release through inhibition of PKCα/NF-κB signaling pathway.
