Effects of Hydroxychloroquine on cell apoptosis in peripheral blood mononuclear cells of systemic lupus erythematosus by PI3K/Akt signaling pathways
10.3969/j.issn.1000-484X.2018.01.019
- VernacularTitle:羟氯喹通过PI3K/Akt通路对系统性红斑狼疮外周血单个核细胞凋亡的作用研究
- Author:
Yu-Fang DAI
1
;
Shi-Fei XIANG
;
San-Shan HE
;
Jiao-E SHENG
;
Wei-Qiong ZHANG
;
Xiao-Dan ZHAO
;
Qing-Chao WU
;
Yang XIANG
;
Lin-Chong SU
Author Information
1. 湖北民族学院附属民大医院风湿科风湿性疾病发生与干预湖北省重点实验室
- Keywords:
Hydroxychloroquine;
Systemic lupus erythematosus;
Peripheral blood mononuclear cells;
Cell apoptosis;
PI3K/Akt signaling pathway
- From:
Chinese Journal of Immunology
2018;34(1):99-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Hydroxychloroquine on cell apoptosis in peripheral blood mononuclear cells of systemic lupus erythematosus and its mechanisms.Methods:The peripheral blood mononuclear cells of 30 active SLE patients and 15 healthy persons were separated for cell culture.There were four groups:control group,SLE group and HCQ 5 mg/L and HCQ 25 mg/L group.MTT was used to measure the inhibitory effect.Annexin V/PI flow cytometry was performed to analyze cell apoptosis.Western blot was used to evaluate the expressions of PI3 K,pAKt,mTOR,BCL-2,BAX and caspase-3.Besides,the PBMCs of SLE patients were treated with HCQ 25 mg/L and the PI3K/Akt pathway inhibitor LY294002 20 μmol/L and its cell growth inhibition and apoptosis were observed.Results:Compared with the control group,the cell growth inhibition and apoptosis rate of SLE patients group were significantly increased(P＜0.05);while the cell growth inhibition and apoptosis rate of HCQ 5 mg/L and 25 mg/L were increased significantly than the SLE patients group(P＜0.05).Compared with SLE patients group,the expression levels of PI3K,pAKt,mTOR and BCL-2 of HCQ group were significantly increased while the expression of BAX and caspase-3 decreased significantly (P＜ 0.05).The PI3K/Akt pathway inhibitor LY294002 could block the PBMCs apoptosis of SLE patients.Conclusion:Hydroxychloroquine can promote the PBMCs apoptosis of SLE patients by PI3K/Akt signaling pathways.
