Levels of Th1/Th2 in spleen lymphocyte of colitic mice without or with Trichinella spiralis infection
10.3969/j.issn.1000-484X.2018.01.014
- VernacularTitle:旋毛虫对不同肠炎模型小鼠脾脏淋巴细胞Th1/Th2水平的影响
- Author:
Ying ZHAO
1
;
Hong-Yue WANG
;
Hai-Chuan DOU
;
Qing-Fei XIAO
;
Yue-Zhu ZHAO
;
Chun-Mei GU
Author Information
1. 吉林大学第一医院人兽共患病教育部重点实验室
- Keywords:
Trichinella spiralis;
Inflammatory bowel disease;
Flow cytometry;
Trinitrobenzesulfonic;
Oxazolone
- From:
Chinese Journal of Immunology
2018;34(1):71-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the levels of Th1 and Th2 in spleen lymphocyte of TNBS and OXZ colitis without or with Trichinella spiralis(T.spiralis)infection.Methods:Female BALB/c mice were randomly divided into 3 groups:50％ Ethanol,TNBS or OXZ,T.spiralis+TNBS or OXZ(at least 6 in each group when mice were killed).The ratio of Th1/Th2 lymphocyte in spleen was detected by FCM with three color intracellular cytokine staining.Results:In spleen lymphocyte of TNBS colitic mice with prior nematode infection,the ratio of Th1/Th2 were not significantly different compared with that seen in colitic mice without prior nematode infection on day 3 post-induction of colitis(P＞0.05),the ratio of Th1/Th2 was significantly lower than which on day 7 post-induction of colitis (P＜0.05).In spleen lymphocyte of OXZ colitic mice with prior nematode infection on days 3 and 7 post-induction of colitis,the ratio of Th1/Th2 was significantly higher than that seen in colitic mice without prior nematode infection(P＜0.05).Conclusion:Infection of mice with T.spiralis distracts the mucosal immune system response from a Th1 response toward a protective Th2 response and up-regulate Tr1-cytokines with an attendant reduction in the severity of colonic damage and balance of Th1/Th2.Prior T.spiralis infection doesn't reduce the severity of OXZ-induced colitis,but without aggravating colitis.
