Preparation of polyclonal antibody and establishment of chemiluminescence immunoassay for aldosterone
10.3969/j.issn.1000-484X.2018.01.013
- VernacularTitle:制备醛固酮多克隆抗体建立检测血浆醛固酮的化学发光免疫分析法
- Author:
Ting-Ting FENG
1
;
Chao-Yi YANG
;
Xue-Feng ZHANG
;
Bin WANG
Author Information
1. 原子高科股份有限公司
- Keywords:
Aldosterone;
Polyclonal antibody;
Biotin-streptavidin system;
Chemiluminescent immunoassay
- From:
Chinese Journal of Immunology
2018;34(1):65-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The polyclonal antibody of aldosterone (ALD) for immunoassay was developed.And a chemiluminescence immunoassay (CLIA) for the determination of ALD in human blood was established.Methods:Aldosterone oxime was prepared by chemical modification and then conjugated with BSA to prepare immunogen.Rabbit anti ALD polyclonal antibody was prepared by immunizing rabbits with the ALD-BSA.The CLIA of ALD was performed using biotin streptavidin amplification system and competition method.Results:After identification,rabbit No.3 received the highest sensitivity to ALD antibody,and the 50％ binding inhibition (IC50) value for ALD concentration was 268 pg/ml.The measuring range of CLIA method using the antibody was 62.5-2 000 pg/ml.The assay sensitivity was 23.7 pg/ml.The intra-and inter-assay coefficients of variation were 6.9％-9.5％ and 8.5％-12.7％,respectively.Analytical recovery rate was in the range of 93.1％-104.1％.The correlation coefficient between measured and expected values were 0.996 after serial dilution.Compared with radioimmunoassay kit,the correlative equation was y =0.932x+4.596,the correlation coefficient was 0.948 (n =95).Conclusion:The result of methodological identification shows that it was in line with the basic requirements of clinical application.
