Immunologic effect of astragalus polysaccharides fermented by probiotic FGM in brain damage mice
10.3969/j.issn.1000-484X.2018.01.007
- VernacularTitle:益生菌FGM发酵黄芪多糖对脑损伤小鼠免疫功能的影响
- Author:
Qing-Hua ZONG
1
;
Yong-Li LOU
;
Hui ZHANG
;
Shi-Shuai WANG
Author Information
1. 郑州大学附属郑州中心医院神经外科
- Keywords:
Probiotic;
Fermented;
Astragalus polysaccharide;
Immunologic function
- From:
Chinese Journal of Immunology
2018;34(1):36-39,43
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immunologic effect of probiotic FGM fermented astragalus membranaceus on brain damage mice.Methods:Astragalus membranaceus was fermented by probiotic FGM,and ethanol subsiding method was used to extract fermented astragalus polysaccharide in fermented astragalus powder.Fermented astragalus polysaccharide 200,100,50 mg/(kg · d) were intraperitoneal injected daily for 7 consecutive days.The brain damage mice model was established at 1 h after the last dose via suture ligation.Spleen index,thymus index were determined by gravimetric method.The proliferation of splenic lymphocytes was determined by MMT method.Enzyme linked immunosorbent assay(ELISA) was used to determine tumor necrosis factor α(TNF-α) and interleukin 1β(IL-1β).Results:Compared with model group,the spleen index and thymus index was significantly increased in fermented astragalus polysaccharide high concentration administration group (P ＜ 0.05).Splenic lymphocyte proliferation was significantly increased (P＜0.05).The content of TNF-α and IL-1 β decreased significantly(P＜0.05).Conclusion:Probiotic FGM fermented astragalus polysaccharide has immune protective effect on brain damage mice.
