Investigation of pathogenesis of IL-17 involved in cytomegalovirus hepatitis
10.3969/j.issn.1000-484X.2018.01.006
- VernacularTitle:IL-17参与巨细胞病毒肝炎致病机制的研究
- Author:
Ling-Ling LIU
1
;
Yuan HUANG
;
Di MA
;
Yi LIAO
;
Xing-Lou LIU
;
Ge LI
;
Sai-Nan SHU
;
Feng FANG
Author Information
1. 华中科技大学同济医学院附属同济医院儿科病毒实验室
- Keywords:
IL-17;
Cytomegalovirus hepatitis;
Immune-pathological injury
- From:
Chinese Journal of Immunology
2018;34(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of pro-inflammatory cytokine IL-17 involved in the pathogenesis of cytomegalovirus hepatitis in vivo.Methods:First of all,disseminated infection model was established.Then,mice were randomly divided into 4 groups:normal control group,MCMV-infected control group,IL-17 blockade group,and isotype control group.Mice were sacrificed on day 7 after infection.The levels of IL-17 protein were detected by Western blot.Hematoxylin eosin (HE) staining was performed to evaluate the pathologic change of the liver.Serum ALT levels were detected by a Roche DPPI biochemical analyzer.The level of serum IL-17 was measured by double antibody sandwich ELISA.The expressions of mRNA of IL-17R,IFN-γand IL-10 in liver were detected by RT-PCR.Results:Compared with MCMV-infected mice and isotype control,the blockade of IL-17 inhibited the expression of IL-17 protein in liver (P＜0.05).The degree of liver damage reduced obviously.The serum ALT was significantly lower [(146±15)vs (102±11)vs (37±12),P＜0.05].The level of serum IL-17 was relatively reduced[(719.76±6.06)vs (722.1±4.62) vs (707.53 ±8.58),P＜0.05].The expression of IFN-γmRNA [(0.56± 0.06)vs (0.55±0.13)vs (0.96±0.2),P＜0.05] and IL-10 mRNA[(0.55±0.073) vs (0.51 ±0.07) vs (0.903 ±0.18),P＜0.05] increased significantly,while that of IL-17R did not change apparently[(0.81±0.16)vs (0.89±0.38) vs (0.87±0.23),P＞0.05].Conclusion:The increased expression of pro-inflammatory cytokine IL-17 is involved in the pathogenesis of immune injury in cytomegalovirus hepatitis.The blockade of IL-17 is helpful to relieve the liver damage and improve the liver function.
