BANCR promotes cell proliferation, invasion and angiogenesis of HepG2
10.3969/j.issn.1000-484X.2018.01.005
- VernacularTitle:BANCR对肝癌细胞HepG2增殖、侵袭和血管新生的促进作用
- Author:
Jing-Jing ZHANG
1
;
Xiang-Peng WANG
;
Xiao-Yu YANG
;
Ying-Hua JI
;
Wen-Yu DI
;
Qing-Qin ZHANG
;
Wei SHEN
;
Hai-Chuan YU
;
Ping LU
Author Information
1. 新乡医学院医学检验学院和河南省分子诊断与医学检验技术协同创新中心
- Keywords:
BANCR;
Human hepatocarcinoma;
Cell proliferation;
Apoptosis;
Invasion;
Acngiogenesis
- From:
Chinese Journal of Immunology
2018;34(1):25-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effects of BANCR on proliferation,apoptosis,invasion and angiogenesis in human hepatocarcinoma cell line HepG2.Methods:The expression of BANCR was detected by quantitative real-time reverse transcription PCR (qRT-PCR).BANCR siRNA and Scramble was respectively transfected into human hepatocarcinoma cell line HepG2.Cell proliferation was detected by CCK-8.Flow cytometry was performed to analyze the apoptosis.Transwell assay was used to test the invasion.Angiogenesis was analyse by tube formation assay.Western blot was executed to check the expression of proliferating cell nuclear antigen (PCNA),caspase-3,matrix metalloprotein 9 (MMP-9),vascular endothelial growth factor(VEGF),acidic fibroblast growth factor (bFGF)and interferon-γ (IFN-γ).Results:The expression of BANCR in HepG2 was higher than L02 (P＜0.05).Compared with control group,the cell proliferation folds in BANCR siRNA was largely decreased.Besides,BANCR siRNA group had a higher apoptosis rate and less invasive cells (P＜0.05).Western blot showed that the expression level of caspase-3 and IFN-γwas obviously enhanced in BANCR siRNA group,and the expression of PCNA,MMP-9,Fn,Vimentin,VEGF and bFGF was distinctly surpressed in BANCR siRNA group compared to control group (P ＜ 0.05).Conclusion:siRNA interference of BANCR promotes apoptosis and represses proliferation,invasion and angiogenesis in human hepatocarcinoma cell line HepG2.
