Distribution of interleukin 17 receptor mRNA in rhesus macaques
10.3969/j.issn.1000-484X.2018.01.004
- VernacularTitle:白介素17受体mRNA在恒河猴中的分布
- Author:
Feng-Jie WANG
1
;
Dong LI
;
Lei YU
;
Yan-Fang CUI
;
Hua-Hu YE
;
Yun-Zhi FA
;
Gui-Bo YANG
Author Information
1. 中国疾病预防控制中心性病艾滋病预防控制中心
- Keywords:
Interleukin 17 receptor;
Real-time PCR;
Tissue distribution;
Rhesus macaques;
African green monkeys
- From:
Chinese Journal of Immunology
2018;34(1):19-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To delineate the distribution and abundance of interleukin 17 receptor mRNA in normal tissues of rhesus macaques.Methods:Tissue samples were collected from different systems including lymphoid system,digestive system and genital system and the mRNA levels of IL-17RA and IL-17RC were examined by real time RT-PCR.Meanwhile the levels of IL-17RA and IL-17RC mRNA in the PBMCs of rhesus macaques,African green monkeys and cynomolgus monkeys were examined.Results:IL-17RA mRNA was expressed in all the tested tissues and the levels were significantly different among these systems (P＜0.05).IL-17RC mRNA level in the large intestine was higher than that in the small intestine.IL-17RA mRNA level in rhesus macaque PBMCs was 3 and 5 times higher than that of cynomolgus monkeys and African green monkeys respectively,while IL-17RC mRNA in the PBMCs was below the limit of detection.Conclusion:IL-17RA is widely distributed in rhesus macaques;the expression level is different among different tissues and species.
