Analysis of immune cells subsets in liver from IκBα-transgenic mice
10.3969/j.issn.1000-484X.2018.01.002
- VernacularTitle:IκBα转基因小鼠肝脏免疫细胞亚群的初步分析
- Author:
Xue-Yao WANG
1
;
Qiu-Ju HAN
;
Jian ZHANG
Author Information
1. 山东大学药学院免疫药物学研究所
- Keywords:
Liver immunity;
IκBα;
NF-κB;
Apoptosis
- From:
Chinese Journal of Immunology
2018;34(1):11-14,24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the liver injury,and the percentage,apoptotic status,cytokine profiles of immune cell subsets in liver from IκBα transgenic mice.Methods:HE staining was performed to detect the liver injury.FACS was used to evaluate the percentage and phenotype of immune cell subsets,including T cells,NK cells and NKT cells,and Annexin V staining was used to evaluate the apoptosis rate of NK and T cells.Furthermore,real-time quantitive PCR was performed to analyze the expression of CCL2,IFN-γ,IL-2 and IL-15.Results:Liver injury was observed in IκBα transgenic mice.The percentage of T cells was lower in liver from IκBα transgenic mice than that in Wild Type(WT) mice,a similar trend was found in NK cells and NKT cells.We also found that NKp46 was inhibited in NK cells from IκBα transgenic mice,accompanied with the increased apoptosis.Also,IFN-γand CCL2 were decreased in IκBα transgenic mice.Conclusion:The percentage,phenotype and cytokine profile of immune cell subsets were affected in IκBα-transgenic mice.
