Susceptibility of Acinetobacter baumannii isolates to eight antimicrobial agents tested by different methods
10.16718/j.1009-7708.2017.06.013
- VernacularTitle:鲍曼不动杆菌对8种常用抗菌药物敏感试验不同方法的比较
- Author:
Hainan WEN
1
;
Shoujun XIE
;
Jianhong ZHAO
;
Qian SUN
;
Honglian WEI
;
Dongyan SHI
;
Zhirong LI
;
Li XU
;
Haifeng WEN
Author Information
1. 承德医学院附属医院检验科
- Keywords:
Acinetobacter baumannii;
E-test;
Kirby-Bauer method;
VITEK
- From:
Chinese Journal of Infection and Chemotherapy
2017;17(6):680-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to analyze the susceptibility profile of Acinetobacter baumannii to antimicrobial agents,and validate the results of different antimicrobial susceptibility testing methods,for improving the quality of antibiotic resistance monitoring data.Methods The susceptibility data of Hebei Provincial Antimicrobial Resistant Investigation Net were analyzed retrospectively and 126 strains ofA.baumannii were collected.The susceptibility ofA.baumannii to piperacillintazobactam,amikacin,gentamicin,ciprofloxacin,levofloxacin,ceftazidime,imipenem,and meropenem was tested by E-test,KirbyBauer method and VITEK system.Results The susceptibility results of the 126 A.baumannii strains showed that the susceptibility to piperacillin-tazobactam,amikacin,gentamicin,levofloxacin and ceftazidime was significantly different between the three methods (P＜0.05).The categorical agreement,major error,minor error,and very major error of Kirby-Bauer method were within acceptable range.There were evident difference in classification consistency for piperacillin-tazobactam,amikacin,levofloxacin between Kirby-Bauer method and VITEK (P＜0.05).Conclusions Different antimicrobial susceptibility testing methods may lead to different results of resistance monitoring data.Bias may be generated in antibiotic resistance surveillance if different methods are used.The susceptibility results of piperacillin-tazobactam,amikacin,levofloxacin derived from VITEK system should be validated by Kirby-Bauer or E-test method.
