Effect of Ultrashort Wave on Macrophage Polarization and Inflammatory Response after Spinal Cord Injury in Rats
10.3969/j.issn.1006-9771.2018.06.003
- VernacularTitle:超短波促进巨噬细胞表型转化并抑制大鼠脊髓损伤后炎症反应的作用
- Author:
Lin GAO
1
;
Zhi-Ping FENG
;
Chen-Fei DAI
;
Zhi-Qiang ZHANG
;
Li-Xin ZHANG
Author Information
1. 中国医科大学附属盛京医院康复中心
- Keywords:
spinal cord injury;
ultrashort wave;
macrophage polarization;
tumor necrosis factor-α;
repair;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(6):634-640
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of ultrashort wave on macrophage polarization and inflammation response after spinal cord injury in rats. Methods A total of 96 Sprague-Dawley rats were randomly divided into sham group, model group and ultrashort wave group, with 32 rats in each group. The spinal cord injury model was established by modified Allen's method in the model group and the ultrashort wave group. The ultrashort wave group received ultrashort wave since the first day after modeling. BBB score was applied to evaluate the locomotion recovery. HE staining was used to as-sess the injury area, while immunochemistry, real-time PCR and Western blotting were applied to measure the mRNA and protein expression of inducible nitric oxide synthase (iNOS), arginase-1 (Arg-1) and tumor necrosis factor-α (TNF-α). Results Compared with the model group, the BBB score increased (P<0.05), the expression of iNOS and TNF-α de-creased, and the expression of Arg-1 increased (P<0.05). Conclusion Ultrashort wave therapy may promote macrophage polarization from M1 to M2, inhibit inflammatory re-sponse and promote recovery after spinal cord injury in rats.
