Effect of Jiawei Naotaifang on Activation of Extracellular Signal-regulated Kinase 1/2 and c-Jun N-terminal Kinase in Ovariectomized Rats after Cerebral Ischemia
10.3969/j.issn.1006-9771.2018.03.005
- VernacularTitle:加味脑泰方对卵巢摘除脑缺血大鼠海马细胞外信号调节蛋白激酶1/2和c-Jun氨基末端蛋白激酶蛋白活化的影响
- Author:
Li-Hua QIN
1
;
Sheng LI
;
Shao-Wu CHENG
;
Lin LIU
;
Yang LIU
;
Juan HUANG
;
Sheng-Qiang GONG
;
Cheng CHENG
;
Jin-Wen GE
Author Information
1. 湖南中医药大学
- Keywords:
cerebral ischemia;
Jiawei Naotaifang;
traditional Chinese medicine;
apoptosis;
extracellular signal-regulat-ed kinase 1/2;
c-Jun N-terminal kinase;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(3):277-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Jiawei Naotaifang on neuronal apoptosis and the mechanism in ovariectomized rats with cerebral ischemia. Methods Female Sprague-Dawley rats(n=40)were randomly divided into sham group(n=10),model group(n=10),es-trogen group(n=10)and Jiawei Naotaifang group(n=10).The model group,estrogen group and Jiawei Naotai-fang group were ovariectomized.Eleven days after ovariectomy,the estrogen group and Jiawei Naotaifang group were given estrogen and Jiawei Naotaifang respectively intragastrically for three days.14 days after ovariecto-my,the model group,estrogen group and Jiawei Naotaifang group were modeled cerebral ischemia with Langa's method.24 hours after modeling,the apoptosis rate of neurons was detected with TUNEL,and the activation of extracellular signal-regulated kinase 1/2(ERK1/2)and c-Jun N-terminal kinase(p-JNK)in hippocampus were de-tected with Western blotting. Results Compared with the model group, the apoptosis rates decreased in Jiawei Naotaifang group and the estrogen group(P<0.001),with more activation of ERK1/2(P<0.01)and less activation of JNK(P<0.01). Conclusion Jiawei Naotaifang can protect neuron from apoptosis by promoting the activation of ERK1/2 and inhibiting the activation of p-JNK.
