Comparison of Inducing Pluripotent Stem Cells to Differentiate into Motor Neuron Precursor in Two Kinds of System
10.3969/j.issn.1006-9771.2018.03.004
- VernacularTitle:两种体系下诱导多潜能干细胞定向分化为运动神经元前体细胞的差异
- Author:
Zhe LI
1
;
Ming-Zhu FANG
;
Hong CHEN
;
Gang-Hua GUO
;
Jia-Hong FAN
;
Zhi-Juan MAO
Author Information
1. 郑州大学第五附属医院
- Keywords:
human-induced pluripotent stem cells;
motor neurons;
neural differentiation
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(3):269-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce human-induced pluripotent stem cells(iPSCs)to differentiate into spinal motor neuron precursor (MNP)and compare the induction efficiency in systems of feeder and feeder-free. Methods iPSCs cultured on mouse feeder cells or in feeder-free condition were induced into neuroepithelial progenitors (NEP) on the sixth day and MNP on the twelveth day.Their morphology was observed under inverted micro-scope,and the markers of iPSCs,NEP,MNP were detected with immunofluorescence.NEP-related genes SOX1 and HOXA3,MNP-related genes OLIG2 and PAX6,and pluripotency genes SOX2 and OCT4 were detected with real-time quantitative polymerase chain reaction. Results iPSCs expressed pluripotency markers,while NEP and MNP expressed high levels of neural related markers and low levels of pluripotency markers in two systems. The expression of the genes SOX1, HOXA3, OLIG2 and OCT4 was higher in the feeder system,and there was no significant difference in the expression of genes SOX2 and PA X 6. Conclusion iPSCs can differentiate into MNP in culture systems of feeder and feeder-free,and the induction efficiency is higher in the feeder system.
