Effect of Interleukin-1β Silence on Expression of Vimentin in Rats with Spinal Cord Contusion

VernacularTitle:沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响
Author: Xi HU ¹ ; Ying-Jie NIU ; Yuan HUANG ; Ying-Jie LI ; Xi ZENG ; Yang XIANG ; Xiao ZHANG ; Nan LIANG
Author Information

1. 成都医学院
Keywords: spinal cord contusion; interleukin-1β; vimentin; siRNA; rats
From: Chinese Journal of Rehabilitation Theory and Practice 2018;24(1):66-70
CountryChina
Language:Chinese
Abstract: Objective To observe the expression of vimentin (Vim) after silence of interleukin-1β (IL-1β) in rats with spinal cord contusion (SCC). Methods The model of SCC was established in 30 Sprague-Dawley rats with Allen's method. The rats were randomized into vector group (n=15) and silence group (n=15), which were injected blank lentivirus vector and vector of IL-1β siRNA, respectively; and divided in three, seven and 28 days subgroups. The relationship between IL-1βand Vim was predicted with GeneMANIA bioinformatics. The expression of Vim protein and mRNA in spinal cord was detected with immunohistochemistry and real-time quantitative polymerase chain reaction. Results GeneMANIA bioinformatic analysis indicated that there was some direct and indirect relationship between IL-1β and Vim. The Vim protein and mRNA expressed in the spinal cord, and was less in the silence group than in the vector group (t>2.875, P<0.05). Conclusion Silence of IL-1β can inhibit the expression of Vim in SCC rats, which may promote the recovery of spinal cord function.