Effect of Electroacupuncture on Learning and Memory and Expression of Beclin-1 in Rats after Cerebral Ischemic-reperfusion
10.3969/j.issn.1006-9771.2018.01.009
- VernacularTitle:电针神庭、百会对脑缺血再灌注大鼠认知功能及 Beclin-1表达的影响
- Author:
Xiao-Dong FENG
1
;
Jing SHI
;
Ming-Yue WAN
;
Cheng-Mei LIU
Author Information
1. 河南中医药大学第一附属医院康复中心
- Keywords:
cerebral ischemia-reperfusion;
electroacupuncture;
learning and memory;
autophagy;
Beclin-1;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the efficacy of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning and memo-ry in rats after cerebral ischemia-reperfusion, and explore the possible mechanism. Methods A total of 45 male Sprague-Dawley rats were randomly divided into sham group (n=15), ischemia group (n=15) and electroacupuncture group (n=15). The left middle cerebral arteries of the latter two groups were occluded with the modified Longa's method for two hours and reperfused, and the electroacupuncture group received elec-troacupuncture at Baihui and Shenting once a day for seven days, then. They were assessed with Longa's score one, three, five and seven days after intervention. They were tested with Morris water maze since four days after intervention, once a day for four days. The cerebral infarction volume was measured with TTC staining seven days after intervention, while the protein expression of Beclin-1 was detected with Western blotting. Results Longa's score improved in the electroacupuncture group compared with that in the ischemia group since three days after intervention (P<0.05). The escape latency decreased and the times cross the area of platform increased in the electroacupuncture group compared with that in the ischemia group at every point (P<0.05). The cerebral infarction volume reduced (F=7.651, P<0.001) and the expression of Beclin-1 decreased (P<0.05) in the elec-troacupuncture group compared with that in the ischemia group.Conclusion Electroacupuncture can improve learning and memory in rats after cerebral ischemia-reperfusion, which may relate with regulation of autophagy network.
