Effects of Triptolide on Expression of Drebrin and Cofilin in Hippocampus of Rats with Alzheimer's Disease
10.3969/j.issn.1006-9771.2018.01.005
- VernacularTitle:雷公藤内酯醇对阿尔茨海默病大鼠海马drebrin和cofilin表达的影响
- Author:
Sai-Sheng ZHANG
1
;
Bao-Lin YANG
;
Li-Xia CHENG
;
Bin WAN
;
Jing NIE
;
Xiao-Ling HU
;
Cheng LÜ
Author Information
1. 南昌大学江西医学院
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
triptolide;
drebrin;
cofilin;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(1):23-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of triptolide on drebrin and cofilin expression in the hippocampus of rats with Alzheim-er's disease (AD). Methods Sixty male Sprague-Dawley rats were equally divided into control group, model group and triptolide-treated group with 20 cases in each group. The AD model was established with unilateral injection of beta amyloid 1-40 (Aβ1- 40) into hippocampus in rats. The control group was established with unilateral injection of normal saline with the same volume into hippocampus in rats. The triptolide-treated group was administered triptolide intraperi-toneally, 0.4 mg/kg, once a day, for 15 days after modeling. Spine density of hippocampal neurons was assayed by Golgi staining. Drebrin and cofilin expression of hippocampal neurons was assayed by immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR). Results The spine density of hippocampal neurons was higher in the triptolide-treated group than in the model group (P<0.05). The average optical density of drebrin was higher in the triptolide-treated group than in the the model group (P<0.01), while the cell number and average optical density of cofilin were lower (P<0.05). The drebrin mRNA expression was higher in the triptolide-treated group than in the model group (P<0.05), and the cofilin mRNA expression was lower (P<0.01). Conclusion Triptolide may delay the degeneration of dendritic spines in hippocampal neurons of AD rats by regulating the expression of drebrin and cofilin.
