Role of cAMP Response Element Binding Protein/Brain-derived Neurotrophic Factor Signaling Pathway in Tiaoxin Formula Improving Learning and Memory of APP/PS1 Mice
10.3969/j.issn.1006-9771.2018.01.003
- VernacularTitle:环磷酸腺苷效应元件结合蛋白/脑源性神经营养因子信号通路在调心方改善APP/PS1小鼠学习记忆中的作用
- Author:
Xiao-Wen WANG
1
;
Hai-Yan DENG
;
Jian WANG
;
Ying XU
Author Information
1. 上海中医药大学
- Keywords:
Alzheimer's disease;
Tiaoxin formula;
Morris water maze;
learning and memory;
cAMP response element binding protein;
brain-derived neurotrophic factor;
signaling pathway;
mice
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(1):11-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of cAMP response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway in the hippocampus of APP/PS1 double transgenic Alzheimer's disease mice, and in-vestigate the effects of Tiaoxin formula on learning and memory. Methods Totally, 54 three-month-old APP/PS1 transgenic mice were randomly divided into model group (n=18), posi-tive control (donepezil) group (n=18) and Tiaoxin formula group (n=18). Another 18 three-month-old C57/BL6 wild mice were chosen as normal group with same strain and same age. All administration groups received rele-vant medicine. Twelve weeks later, Morris water maze test was used to test the behavior. The real-time quantita-tive polymerase chain reaction (RT-qPCR) was used to detect the expression of CREB mRNA and BDNF mRNA in hippocampus. Western blotting was used to detect the expression of phosphorylated CREB (p- CREB) and BDNF in hippocampus. Results Compared with the model group, the escape latency significantly shortened (P<0.01), the percentage of staying time in the quadrant of original platform significantly increased (P<0.05), the expression of CREB mRNA and BDNF mRNA in hippocampus significantly increased (P<0.05), and the expression of p-CREB and BDNF pro-tein in hippocampus significantly increased (P<0.01) in Tiaoxin formula group. Conclusion Tiaoxin formula could improve the spatial learning and memory of APP/PS1 double transgenic mice with AD, which might be related to the up-regulation of CREB/BDNF signaling pathway.
