Effects of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glycoside on Senile Plaques Formation and Inflammatory Response in APP/PS1 Mice
10.3969/j.issn.1006-9771.2018.01.002
- VernacularTitle:二苯乙烯苷对APP/PS1小鼠老年斑形成和炎症反应的影响
- Author:
Rui HUANG
1
;
Cui-Cui YANG
;
Lin LI
;
Lan ZHANG
Author Information
1. 首都医科大学宣武医院药物研究室
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
2;
3;
5;
4'-tetrahydroxy-stilbene-2-O-β-D-glycoside;
learning and memory;
beta amyloid;
senile plaques;
inflammation;
mice
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(1):2-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glycoside (TSG) on formation of senile plaques and beta amyloid (Aβ), as well as activation of microglia and astrocytes, in cortex and hippocampus of APP/PS1 double transgenic mice. Methods A total of 64 five-month-old APP/PS1 mice were randomly divided into model group (n=16), low-dose TSG (0.05 g/kg) group (n=16), high-dose TSG (0.1 g/kg) group (n=16), and donepezil group (n=16); other 32 same age wild type (WT) mice were randomly divided into normal control group (n=16) and high-dose TSG (0.1 g/kg) WT group (n=16). The normal control group and model group were given distilled water, and the other groups were given the corresponding drugs intragastrically. The mice were tested with object recognition test, the deposi-tion of plaques in brain was detected with Congo red staining, and the expression of Aβ40/42, ionized calcium bind-ing adapter molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) with immunohistochemistry after seven months of treatment (twelve-month-old). Results Compared with the model group, the discrimination index significantly increased (P<0.01), the deposition of plaques decreased in brain (P<0.05), and the expression of Aβ40/42, Iba1 and GFAP all significantly decreased in each treatment group (P<0.05). Conclusion TSG can improve learning and memory of APP/PS1 transgenic mice, reduce Aβ deposition and senile plaques, and reduce the inflammatory response, even in low-dose, which is similar to that of donepezil.
