Targeting ability and photoacoustic imaging of novel nanoparticle probe loaded with ZnPc and docetaxel: An in vitro study
10.13929/j.1672-8475.201711037
- VernacularTitle:载多烯紫杉醇及酞菁锌并靶向乳腺癌多功能分子探针体外光声显像特性及寻靶能力
- Author:
Tao YU
1
;
Xiaoling HUANG
;
Yang CAO
;
Yuli CHEN
;
Haitao RAN
Author Information
1. 重庆医科大学附属第一医院超声科
- Keywords:
Breast neoplasms;
Photoacoustic imaging;
Zinc phthalocyanine;
Targeted nanoparticle probe
- From:
Chinese Journal of Interventional Imaging and Therapy
2018;15(5):295-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the targeting ability and photoacoustic imaging of novel nanoparticle probe loaded with ZnPc and docetaxel.Methods The polymeric nanoparticles probe loaded with ZnPc and docetaxel were fabricated using double emulsion method.RGDfK was modified on the surface for breast carcinoma targeting by carbodiimide method.The encapsulating ratio (ER) and drug loading (DL) of ZnPc and docetaxel were assessed.The modification rate and targeting ability of molecular probe were tested in vitro,and the photoacoustic imaging and drug release profiles were observed.Results The probes were loaded with ZnPc and docetaxel efficiently and successfully.The size of novel nanoparticle loaded with ZnPc and docetaxel was (266.00 ± 65.85)nm,and the surface potential was (-29.20± 6.27)mV.ER and DL of docetaxel was (88.00±0.32)％ and (34.92±0.02)μg/mg,of ZnPc was (97.25±0.22)％ and (30.87±0.11)μg/mg,respectively.The probes had certain sustained slow release effect and showed obvious photoacoustic signals,which enhanced with the increase of the content of ZnPc.Flow cytometry detection results showed that the RGDfk modification rate was 89.19％.The apoptotic rate of novel nanoparticle loaded with ZnPc and docetaxel targeting breast carcinoma increased after the laser irradiation in vitro.Conclusion The new polymeric multifunctional nanoparticles probe has an ideal size and good photoacoustic signals,also the ability to target breast carcinoma cells and inhibit the proliferation of breast carcinoma cells efficiently.
