Preparation and in vitro ultrasound/photoacoustic imaging of folate receptor-targeted, doxorubicin and melanin-loaded multifunctional contrast media
10.13929/j.1672-8475.201710002
- VernacularTitle:制备叶酸受体靶向载阿霉素/黑色素多功能造影剂及体外超声/光声显像
- Author:
Yuanzhi YAO
1
;
Zhigang WANG
;
Liang ZHANG
;
Dong WANG
;
Fang LI
Author Information
1. 重庆市肿瘤医院重庆市肿瘤研究所重庆大学附属肿瘤医院超声医学科
- Keywords:
Ultrasonography;
Photoacoustic imaging;
Contrast media;
Doxorubicin;
Melanin
- From:
Chinese Journal of Interventional Imaging and Therapy
2018;15(5):286-290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media,and to observe its characteristics,targeting,imaging performance and cell toxicity on human MDA-MB-231 cell lines in vitro.Methods The folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media wasprepared using single emulsion method and freeze-drying and C3 Fs-inflation.Its size and drug loading efficiency were measured with Malvern instrument and UV-visible spectrophotometry.Its enhanced respectively ultrasound and photoacoustic imaging were observed in vitro model.The targeting performance of contrast media and its cell toxicity werechecked after co-incubated with human MDA-MB-231 cell lines.Results The average diameter of contrast media was (659.60±27.56)nm,Zeta potential was (-38.90±4.00)mV,and the drug loading capacity was 85.72 μg/mg.In vitro,the targeting experiment showed that a large number of folate receptor-targeted contrast media were aggregated and adhered firmly to the MDA-MB-231 cells.The folate receptor-targeted contrast media significantly inhibited the proliferation of human MDA-MB-231 cells.The ultrasound and photoacoustic imaging of folate receptor-targeted contrast media were significantly enhanced.Conclusion The folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media is a set of targeting treatment,enhancing ultrasound and photoacoustic imaging,having potential of perfect molecular probe for targeting imaging and treatment of human breast cancer.
