miR-128 affects biological behavior of breast cancer cells through the targeting of RECK
10.11659/jjssx.03E018001
- VernacularTitle:miR-128通过RECK靶向作用影响乳腺癌细胞的生物学行为
- Author:
Yan-Jun YE
1
;
Fu-Jun HAO
;
Wei CHEN
;
Ling ZHANG
Author Information
1. 宝鸡市人民医院普外科
- Keywords:
breast cancer;
miR-128;
RECK;
cell cycle;
apoptosis;
MCF-7 cells
- From:
Journal of Regional Anatomy and Operative Surgery
2018;27(10):707-710
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological behavior of breast cancer cells induced by miR-128 targeting RECK and its possible mechanism.Methods The expression of miR-128 in cell lines and transfection efficiency of lentiviral were detected by Western blot.Flow cytometry was used to detect the effect of miR-128 on the apoptosis of breast cancer cells.The dual luciferase assay was used to detect the in-teraction of miR-128 and RECK in breast cancer cells.Flow cytometry was used to detect the reversal effect of RECK on miR-128 in the cell cycle and colony formation of breast cancer cells.Results The expression of miR-128 in MCF-7 cells was 3.05 times that of NC cells,and the lentiviral transfection efficiency was obvious.miR-128 inhibits the growth of MCF-7 cells by inducing apoptosis and inhibiting cell cycle progression.RECK was the direct target of miR-128,and miR-128 directly regulated the expression of RECK.RECK ectopic recovery,miR-128 on breast cancer cell apoptosis and cell cycle inhibition had also been partially restored.Conclusion miR-128 plays an important role in the development and progression of breast cancer.It can interact with RECK to regulate the biological behavior of breast cancer cells,which suggests that miR-128 and RECK may be the potential therapeutic targets of breast cancer.
