Effects of bletilla striata polysaccharideon on expression of vascular endothelial growth factor in rat ovaries and malondialdehyde in rat serum and endometrial morphology during perimenopause period
10.11659/jjssx.05E018120
- VernacularTitle:白芨多糖对围绝经期大鼠卵巢VEGF、血清MDA、子宫内膜形态学的影响
- Author:
Wen-Bin YAN
1
;
Xiang-Tao ZENG
;
Meng-Shi DAI
;
Hang YE
;
Yuan FANG
;
Chun-Xia HUANG
Author Information
1. 长沙医学院基础医学院
- Keywords:
bletilla striata polysaccharide;
VEGF;
MDA;
endometria;
perimenopause period;
ovary
- From:
Journal of Regional Anatomy and Operative Surgery
2018;27(10):698-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the bletilla striata polysaccharide on the expression of ovarian vascular endothelial growth factor(VEGF) and serum malondialdehyde(MDA)and endometrial morphology in rat during perimenopause period.Methods The animal model of perimenopause rats was established by unforced aging.A total of 40 female SD rats(12~14 months old)were randomly divid-ed into four groups:Chinese medicine low(0.5 g/kg),medium(1 g/kg),high(2 g/kg) dosage group and the elder model group.The rats were given with corresponding drugs for 8 weeks.The content changes of ovarian VEGF protein was detected by western blot;serum MDA con-tent was detected by thiobarbituric acid;the morphology of endometrium in rats was observed by HE staining.Results Eight weeks later, compared with the elder model group,the VEGF protein expression in Chinese medicine low,medium,high dosage group increased,the serum MDA content in Chinese medicine low,medium,high,dosage group reduced,the differences were statistically significant( P<0.01),with dose dependence.Endometrial thickening and glandular increasing with the increase of dose.Conclusion Bletilla striata polysaccharide can upregulate the expression of VEGF protein,and down regulate the level of serum MDA,which improves the uterine function so as to delay the process of perimenopause in rats.
