Effects of miR-181 b on proliferation and apoptosis of thyroid papillary carcinoma cells by down-regulating CYLD protein
10.11659/jjssx.12E017078
- VernacularTitle:miR-181b通过下调CYLD蛋白影响甲状腺乳头状癌细胞的增殖和凋亡
- Author:
Lei WANG
1
;
Dong-Sheng YU
;
Lin-Xiao ZHAO
;
Li-Xia CHAI
Author Information
1. 延安大学咸阳医院病理科
- Keywords:
miR-181b;
thyroid papillary carcinoma;
CYLD;
apoptosis
- From:
Journal of Regional Anatomy and Operative Surgery
2018;27(5):322-325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-181b on the proliferation and apoptosis of thyroid papillary carcinoma cells by down-regulating CYLD protein and its mechanism. Methods qPCR was used to detect the expression and difference of miR-181b in papilla-ry thyroid carcinoma and normal thyroid tissue. Western blotting was used to detect the regulation between miR-181b and CYLD protein. Clone formation assay was used to detect the proliferation of thyroid cancer cells after inhibition of miR-181b. Flow cytometry was used to detect the apoptosis of thyroid carcinoma cells after inhibition of miR-181b. Results Compared with normal thyroid tissue,the expression of miR-181b in papillary thyroid carcinoma was up-regulated,the difference was statistically significant(P<0. 05). The expression level of miR-181b in FTC-133 cell line was relatively high. Western blotting confirmed that miR-181b could directly regulate the expression of CYLD protein. Inhi-bition of miR-181b expression can inhibit the proliferation of thyroid cancer cells,and to some extent promote its apoptosis behavior. Conclu-sion miR-181b can regulate the expression of CLYD and affect the proliferation and apoptosis of thyroid cancer cells.
