Expression of Notch signaling pathway in cultured human osteoarthritic articular chondrocytes
10.11659/jjssx.08E017010
- VernacularTitle:Notch信号通路在骨关节炎软骨细胞中的表达及意义
- Author:
Qing ZHANG
1
;
Ming XIANG
;
Hang CHEN
;
Guo-Yong YANG
;
Xiao-Chuan HU
;
Yi-Ping LI
;
Fei-Xu PAN
;
Ming-Yue DENG
Author Information
1. 四川省骨科医院上肢科
- Keywords:
DLL4;
DAPT;
osteoarthritis;
cartilage cell
- From:
Journal of Regional Anatomy and Operative Surgery
2018;27(4):240-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of Notch signaling pathway in the matrix synthesis of osteoarthritis cartilage cell and its possi -ble mechanism.Methods Selected the femoral condylar cartilage of 8 patients who were admitted into our hospital and treated with knee joint replacement as the observation group,while the normal femoral condyle cartilage of one patient with above-knee amputation was selected as the control group.The specimens were given histological examination or cell isolation culture and detection.The cultured cells were divided into 4 groups,namely the normal cartilage cells,OA cartilage cells,OA cartilage cells with γ-secretase inhibitor DAPT,OA cartilage cells with recombinant human proteins Delta4.Then immunohistochemistry and Western blot were used for detecting the expression of Notch -1,Notch-3, Jagged-1,Jagged-2 and HES5 in chondrocytes in vitro.Results The expression of Notch signaling pathway and the phenotype of cartilage cells changed in the osteoarthritis.The expression of Notch-1,Jagged-1,Jagged-2 and HES5 were activated.γ-secretase inhibitor DAPT (20 μmol/L)could inhibit the expression of Notch-1,HES5,Jagged-1,and Notch-3,while it had no obvious effect on the expreesion of Jag-ged-2.Recombinant human proteins Delta 4(100 ng/μL)could promote the expression of Notch-1,Jagged-2,and HES5,and it had no obvi-ous effect on the expreesion of Jagged-1 and Notch-3.Conclusion The expression of Notch signaling pathway of cartilage cells changed in the osteoarthritis.DLL4 can activate the Notch signaling pathway and DAPT can inhibit the Notch signaling pathway.
