Effect of hypoxia on proliferation and invasion of non-small-cell lung cancer cell line A549
- VernacularTitle:低氧微环境对非小细胞肺癌细胞系A549细胞增殖和侵袭的影响
- Author:
Fan YANG
1
;
Chao ZHANG
;
Guang-Da ZENG
;
Jian WANG
;
Dao-Yuan GUO
;
Wei XIONG
Author Information
- Keywords: lung cancer; hypoxia; Wnt/β-catenin; proliferation; invasion
- From: Journal of Regional Anatomy and Operative Surgery 2018;27(3):173-178
- CountryChina
- Language:Chinese
- Abstract: Objective To study the effect of hypoxia on proliferation and invasion in non-small-cell lung cancer cell line A549 and its molecule mechanism.Methods The non-small-cell lung cancer cell line A549 were stimulated under hypoxia and normoxia for 24 hours in vitro.The proliferation ability was detected by CCK8.The invasion ability was detected by Transwell assay.The mRNA and protein expression levels of HIF-1 oα and β-catenin were detected by RT-PCR and Western blot.Furthermore,β-catenin siRNA was used to investigate the role of β-catenin on hypoxia induced proliferation and invasion.Results The results demonstrated that hypoxia could promote A549 cells proliferation and invasion.The mRNA and protein level of HIF-1oα and β-catenin was upregulated by hypoxia treatment,whereas β-catenin siRNA could block these processes.Conclusion Hypoxia can promote the proliferation and invasion of non-small-cell lung cancer cell line A549 by activating Wnt/β-catenin signaling pathway.
