Mechanism of IL-6 induced secretion of IL-10 in nasopharyngeal carcinoma CNE-2 cells
10.11659/jjssx.11E017061
- VernacularTitle:IL-6诱导鼻咽癌CNE-2细胞分泌IL-10的机制研究
- Author:
Wang-Bo YU
1
;
Qing-Chun PAN
;
Si-Jun LI
;
Wan LI
;
Li-Fang MAO
Author Information
1. 川北医学院附属医院耳鼻咽喉科
- Keywords:
interlukin-6;
interlukin-10;
NF-κB;
nasopharyngeal carcinoma
- From:Journal of Regional Anatomy and Operative Surgery
2018;27(3):163-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of IL-6 stimulated the secretion of IL-10 in nasopharyngeal carcinoma CNE-2 cells.Methods Nasopharyngeal carcinoma CNE-2 cells were cultured with recombination cytokine IL-6 in vitro,or anti-IL-6 receptor antibody and signal pathway inhibitor were pre-incubated for 1 hour,and then IL-6 were added,the mRNA and protein level of IL-10 were separately detected by RT-PCR and enzyme-linked immunosorbent assay.Results Compared with the control group,the expression and secretion of IL-10 in IL-6 stimulated group were significantly increased,which was in a dose and time dependent way,the difference was significant(P ＜ 0.01).Additionally,IL-6 stimulated the expression and secretion of IL-10 by CNE-2 cells were significantly decreased in following pre-incubated with anti-IL-6 receptor antibody or NF-κB inhibitor,the difference was significant(P ＜ 0.01),but such effect was not detected when CNE-2 cells were pre-incubated with the PI3K inhibitor,p38/MAPK inhibitor,JNK inhibitor,MEK1/2 inhibitor and STAT3 inhibitor.Conclusion IL-6 can induce the expression and secretion of IL-10 in nasopharyngeal carcinoma CNE-2 cells via IL-6R/NF-κB signal pathway,and blocking IL-6 signal may be useful for the immunotherapy of nasopharyngeal carcinoma.
