Preliminary study on adverse effects and mechanism of germ cells induced by Glyphosate

VernacularTitle:草甘膦致生殖细胞毒性效应及机制的初步研究
Author: Wen-Long ZHANG ¹ ; Xiao JIANG ; Li YIN ; Ning ZHANG ; Hong-Qiang CHEN ; Bang-Jing MA ; Jia CAO ; Jin-Yi LIU
Author Information

1. 第三军医大学军事预防医学系毒理学研究所
Keywords: Glyphosate; apoptosis; cell cycle; germ cells
From: Journal of Regional Anatomy and Operative Surgery 2017;26(11):781-785
CountryChina
Language:Chinese
Abstract: Objective To study the effects and mechanism of Glyphosate induced cell cycle arrest and apoptosis in mouse spermatogenic cells(GC-2).Methods Morphology of the GC-2 cells was observed by optical microscope and Transmission Electron Microscopy.Flow cytometry were used to detect cell cycle arrest and apoptosis.Cell cycle arrcst and apoptosis related proteins were detected by Western blot.Results Glyphosate could inhibit cell proliferation and impair endoplasmic reticulum and mitochondria significantly,which was associated with G1 phase arrest and apoptosis in GC-2 cells.Glyphosate lead to cell cycle arrest and apoptosis by regulating the expressions of G1/S phase checkpoint regulatory factor proteins such as p21,CyclinD1,CyclinE and activating caspase3.Conclusion Glyphosate has adverse effects on male germ cells by inducing cell cycle arrest and apoptosis.