Effects of Di ao Xin xue Kang on connexin 43 of myocardial gap junction after acute ischemia injury
10.3760/cma.j.issn.1008-6706.2018.12.019
- VernacularTitle:地奥心血康胶囊对大鼠急性缺血心肌间隙连接蛋白43的影响
- Author:
Dianye WANG
1
;
Zhigeng HU
Author Information
1. 山西中医学院附属医院心病科
- Keywords:
Myocardial ischemia;
Arrhythmias;
cardiac;
Ischemic preconditioning;
myocardial;
Connexin 43;
Di Ao Xin Xue Kang
- From:
Chinese Journal of Primary Medicine and Pharmacy
2018;25(12):1569-1572
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Di ao Xin xue Kang on connexin 43 ( Cx43 ) , in order to make clear the protective mechanism against ischemia -induced arrhythmias .Methods The rats were randomly divided into 3 groups according to the digital table:SM operation group(group SM,n=10),ischemia group(group IM,n=10),Di ao Xin xue Kang group(group DA,n=10).Myocardial infarction was induced by legation the left anterior descending coronary artery for 120 min.The expression of Cx43 of the ischemic myocardium was studied by immunohistochemistry technique .The ventricular arrhythmia was observed , and MDA, SOD levels were measured . Results Compared with the SM group ,the arrhythmia score[(3.8 ±0.7)points vs.(0.7 ±0.2)points,t=13.466, P<0.01]and serum MDA level[(8.3 ±0.8)nmol/mL vs.(4.2 ±0.7)nmol/mL,t=12.197,P<0.01]in the IM group were significantly increased,SOD activity in the IM group[(68.2 ±5.5)U/mL vs.(144.1 ±7.9)U/mL,t=24.932,P<0.01]decreased,and serum Cx43 content[(0.39 ±0.06) vs.(0.92 ±0.05),t=21.459,P<0.01] significantly decreased.Compared with IM group,the arrhythmia score of the DA group [(2.6 ±0.8) vs.(3.8 ± 0.7),t=3.570,P<0.01]decreased,and serum Cx43 content[(0.60 ±0.08) vs.(0.39 ±0.06),t=6.641,P<0.01]increased and distributed regularly.Conclusion Di ao Xin xue Kang can effectively alleviate Cx43 degrading induced by acute myocardial infarction ,the mechanism of this effect is associated with resisting oxidative stress .
