Clone correlation of carbapene m-resistant Acinetobacter baumannii in en-vironment of intensive care units in Guangzhou City
10.3969/j.issn.1671-9638.2018.04.004
- VernacularTitle:广州市ICU环境中耐碳青霉烯类鲍曼不动杆菌的克隆相关性
- Author:
Yun-Wan LIN
1
;
Yong ZHOU
;
Xu ZHANG
;
Xiao-Ning LI
;
Zheng-Yang ZHAO
;
Yuan LIU
Author Information
1. 广州市疾病预防控制中心
- Keywords:
Acinetobacter baumannii;
clone correlation;
carbapenemase gene;
pulsed-field gel electrophoresis;
multilocus sequence typing
- From:
Chinese Journal of Infection Control
2018;17(4):294-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the clone correlation of carbapenem-resistant Acinetobacter baumannii (CRAB)in the environment of intensive care units(ICUs)in Guangzhou City,identify genotypes,and provide basis for prevention and control of healthcare-associated infection(HAI).Methods 39 strains of CRAB isolated from en-vironment of ICUs in 7 hospitals in Guangzhou City were collected,susceptibility to 10 kinds of antimicrobial agents was detected by Kirby-Bauer method,OXA gene of strains was detected by polymerase chain reaction(PCR),clone polymorphism analysis was performed with pulsed-field gel electrophoresis(PFGE)and multilocus sequence typing (MLST).Results Among 39 strains of CRAB,resistance rate to levofloxacin was the lowest(56.4%),resistance rates to other 9 antimicrobial agents were all>90%. PCR results showed that 39 strains(100%)of CRAB all car-ried OXA-51 gene,37(94.9%)carried OXA-23 gene,but OXA-24 and OXA-58 genes were not found. PFGE showed that 38 CRAB strains were divided into 5 clones,group A was the main epidemic clone,MLST analysis showed that the main clone of CRAB was ST195.Conclusion Transmission of CRAB clone carrying OXA-23 gene exists in the ICU environment of Guangzhou City,cleaning and disinfection of ICU environment should be intensi-fied,so as to reduce HAI caused by CRAB.
