Relationship between STING gene expression and HBV DNA load in peripheral blood mononuclear cells of patients with chronic hepatitis B
10.3969/j.issn.1671-9638.2018.03.003
- VernacularTitle:慢性乙型肝炎患者外周血单个核细胞中STING基因表达与HBV DNA载量的关系
- Author:
Xiao-Yan YANG
1
;
Ping-An ZHANG
;
Zhi-Li NIU
;
Fang-Ping WANG
Author Information
1. 武汉大学人民医院
- Keywords:
chronic hepatitis B;
stimulator of interferon gene;
viral load;
interferon type Ⅰ
- From:
Chinese Journal of Infection Control
2018;17(3):196-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect mRNA expression of stimulator of interferon genes(STING)and type Ⅰ interferons(IFN-α and IFN-β)in peripheral blood mononuclear cells(PBMCs)of patients with chronic hepatitis B(CHB), and evaluate its correlation with hepatitis B virus load.Methods 88 untreated CHB patients(CHB group)and 74 healthy persons(control group)who performed physical examination were chosen from Renmin Hospital of Wuhan University during the same period between February 2016 and February 2017.Expressions of mRNA of STING, IFN-α, and IFN-βwere detected by quantitative real-time polymerase chain reaction(PCR), their relative expression values were obtained by 2-ΔΔCT method, results were statistically analyzed.Results The expression of STING, IFN-α, and IFN-βmRNA in peripheral blood of CHB patients were 2.95, 3.14, and2.01folds of healthy controls respectively, differences were statistically significant(t=-4.72, -3.41, -2.31, respectively, all P＜0.05).STING relative expression in patients with HBV DNA load≤104 IU/mL was 2.98, 3.76, and 3.97 folds of patients with HBV DNA load 104-105 IU/mL, 105-106 IU/mL, and＞106 IU/mL, respectively(P＜0.05).mRNA expressions of STING in CHB patients were positively correlated with that of IFN-αand IFN-βmRNA (r=0.475, 0.503, respectively, both P＜0.05).Conclusion The expression of STING increased in patients with CHB, high expression of STING impacted the replication of HBV.
