BBI blocks LPS-mediated inhibitory effect on tight junction protein of intestinal epithelial cells
10.3969/j.issn.1671-9638.2018.03.001
- VernacularTitle:BBI阻断LPS对肠道上皮细胞间紧密连接蛋白的抑制作用
- Author:
Jun GU
1
;
Jin-Biao LIU
;
Wen-Zhe HUO
Author Information
1. 武汉大学基础医学院
- Keywords:
Bowman-Birk inhibitor;
BBI;
intestinal epithelial cell;
tight junction protein;
TLR4;
NF-κB
- From:
Chinese Journal of Infection Control
2018;17(3):185-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the blocking effect and mechanism of Soybean-derived Bowman-Birk inhibitor (BBI)on LPS-mediated downregulation for tight junction protein(HT-29 cells)in intestinal epithelial cells(IECs). Methods The toxic effect of LPS and BBI on HT-29 cells was detected by CCK8 Kit.HT-29 cells were pretreated by BBI for 6 hours prior to LPS stimulation, the expression of tight junction protein(ZO-1 and Occludin), TLR4, and MyDD8 was detected by the quantitative real-time polymerase chain reaction(PCR)and Western Blot;activation of NF-κB was measured by Western Blot.Results LPS(1 000ng/mL)and BBI(1 000μg/mL)showed no cytotoxicity on HT-29 cells.LPS could significantly upregulate the expression of TLR4 in HT-29 cells, the up-regulation had time-dose effect, and could significantly downregulate the expression of tight junction protein, the down-regulation effect was directly proportional to the concentration of LPS, could activate NF-κB, and had dose effect, effect of LPS on HT-29 cells could be significantly inhibited by BBI.Conclusion By inhibiting the expression of TLR4 and activation of NF-κB in IECs induced by LPS, BBI can significantly block the LPS-mediated inhibitory effect on tight junction protein in intestinal epithelial cells.
