Carriage of drug resistance genes and homology of carbapenem-resistant Acinetobacter baumannii isolated from respiratory intensive care unit
10.3969/j.issn.1671-9638.2018.01.004
- VernacularTitle:呼吸ICU耐碳青霉烯类鲍曼不动杆菌耐药基因的携带情况及同源性
- Author:
Tian-Jiao LI
1
;
Tao HUANG
;
Hua WU
;
Yu SU
;
Sheng-Miao FU
;
Hui-Qun FU
;
Xu-Ming WANG
;
Wen-Fang LONG
Author Information
1. 海南省人民医院
- Keywords:
carbapenemase-resistant;
Acinetobacter baumannii;
drug resistance gene;
homology analysis
- From:
Chinese Journal of Infection Control
2018;17(1):16-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate drug resistance genes and epidemic characteristics of β-lactamase carried by carbapenem-resistant Acinetobacter baumannii (CRAB) in the respiratory intensive care unit(RICU) in a hospital.Methods Clinically isolated CRAB from RICU patients in October-December 2015 were collected.Five drug resistance genes (KPC-2,IMP,VIM,NDM-1,OXA-23) were specifically amplified by polymerase chain reaction (PCR),amplified products were performed agarose gel electrophoresis and sequencing analysis,the homology was analyzed with pulsed-field gel electrophoresis (PFGE).Results A total of 22 CRAB strains were isolated in October-December 2015,19 (86.36%) of which were isolated from sputum.The resistance rate of 22 CRAB strains to compound sulfamethoxazole was 59.09 %,resistance rate to minocycline was 9.09 %,all were sensitive to polymyxin B,resistance rates to other antimicrobial agents were more than 80%.Three kinds of resistance genes KPC-2,IMP and NDM-1 were not found by PCR amplification,positive rates of VIM and OXA-23 were both 100%.PFGE homology analysis revealed that 22 strains were divided into 13 different types,each type contained 1-5 strains,9 types(69.23%) contained only 1 strain respectively,the other 4 types (30.77%) contained 2-5 strains.A5,A7,and A8;A9,A11,A14,A19 and A22;A4,A10 and A12;A16 and A18 were of the same type respectively.Conclusion The main types of β-lactamase-resistant genes of CRAB in RICU are VIM and OXA-23.Homology analysis shows a small parts are of the same clone strains,which reveals epidemic of a small scale.