Effect of Acinetobacter baumannii biofilm on antimicrobial resistance
10.3969/j.issn.1671-9638.2018.01.001
- VernacularTitle:鲍曼不动杆菌生物被膜对抗菌药物耐药性的影响
- Author:
Fei LIN
1
;
Bing-Jie DU
;
Can GAO
;
Bao-Dong LING
Author Information
1. 成都医学院结构特异性小分子药物研究四川省高校重点实验室
- Keywords:
Acinetobacter baumannii;
biofilm;
glass tube;
polypropylene plastic;
tigecycline;
polymyxin;
drug resistance
- From:
Chinese Journal of Infection Control
2018;17(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand biofilm-forming ability of Acinetobacter baumannii(A.baumannii) in clinical isolates,and evaluate the effect of biofilm formation on antimicrobial resistance.Methods A total of 47 A.baumannii clinical isolates were collected,biofilm-forming ability was detected by crystal violet staining assay,the minimal inhibitory concentration(MIC) of 12 kinds of antimicrobial agents to biofilrn forming isolates were detected when the isolates were in planktonic or biofilm formatted condition;biofilm-forming ability of positive isolates at different culture condition (shaking or stable) and on different materials(glass tube or polypropylene plastic centrifugal tube) were detected.Results Thirteen isolates (27.66％) exhibited biofilm formation.In planktonic condition,antimicrobial resistance rates of these isolates were all ＜35％,no isolates shown resistance to polymyxin and tigecycline,in biofilm formatted condition,resistance rates to tigecycline was 15.38％,resistance rates to the other antimicrobial agents were all 100％.Of 13 tested isolates,≥10 isolates shown higher absorbance in the shaking condition than in the stable condition,≥10 tested isolates shown higher absorbance in polypropylene plastic centrifugal tube than in the glass tube.Conclusion The resistance of A.baumannii to antimicrobial agents is greatly changed after the formation of biofilm,which may results in the failure of antimicrobial therapy for infection.
