Partial purification and characterization of a novel murine factor that augments the expression of class I MHC antigens on tumor cells.
- Author:
Niti PURI
1
;
R K SAXENA
Author Information
1. School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
MHC class I;
IFN-g;
MHC-AF;
Tumor cells;
Cytokines
- MeSH:
Animal;
Antibodies/pharmacology;
Chymotrypsin/metabolism;
Chymotrypsin/chemistry;
Comparative Study;
Concanavalin A/pharmacology;
Heat;
Histocompatibility Antigens Class I/metabolism*;
Histocompatibility Antigens Class I/drug effects;
Interferon Type II/pharmacology;
Interferon Type II/metabolism;
Interferon Type II/immunology;
Lymphocytes/physiology;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Proteins/pharmacology*;
Proteins/metabolism;
Proteins/isolation & purification*;
Spleen/cytology;
Trypsin/metabolism;
Trypsin/chemistry;
Tumor Cells, Cultured/immunology;
Tumor Cells, Cultured/drug effects
- From:Experimental & Molecular Medicine
1998;30(2):93-99
- CountryRepublic of Korea
- Language:English
-
Abstract:
A soluble factor which augments the expression of major histocompatibility complex class I (MHC I) antigens on a number of murine tumor cell lines, has been isolated from the culture supernatants of mixed lymphocyte reaction of spleen cells derived from C57B1/6, Balb/c and Swiss mice. The factor, termed MHC-augmenting factor (MHC-AF) has been partially purified by Sephadex G-100 column chromatography and reverse phase HPLC. MHC-AF activity is associated with an 18 kDa molecule. MHC-AF activity was resistant to pH 2.0 treatment and partially purified MHC-AF preparations did not have any activity in L929 cell/vesicular stomatitis virus (VSV) interferon bioassay system. Antibodies to IFN-gamma did not block the activity of MHC-AF. These results indicate that a MHC-AF distinct from IFN-gamma, is produced by mouse spleen cells undergoing a mixed lymphocyte reaction.
