Molecular biological characterization of enterovirus variant isolated from patients with aseptic meningitis.
- Author:
Yong Tae JUNG
1
;
Gum Ryong KIM
;
Soon Young PAIK
Author Information
1. Department of Microbiology, College of Medicine, Catholic University of Korea, Seoul.
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Aseptic Meningitis;
enterovirus;
RT-PCR;
RFLP assay
- MeSH:
5' Untranslated Regions;
Base Sequence;
Capsid/genetics;
Comparative Study;
Cysteine Endopeptidases/genetics;
Echovirus 9/genetics*;
Genome, Viral;
Human;
Meningitis, Aseptic/virology*;
Molecular Sequence Data;
RNA Helicases/genetics;
Variation (Genetics)*
- From:Experimental & Molecular Medicine
1998;30(2):101-107
- CountryRepublic of Korea
- Language:English
-
Abstract:
In Korea, there was a big outbreak of aseptic meningitis in 1993. Six clinical isolates of enterovirus were obtained from patients with aseptic meningitis and were identified as echovirus type 9 by serotyping with a pool of neutralizing antisera. For molecular characterization of the isolates, the nucleotide sequences of 5'-noncoding region (NCR), VP4, VP2, VP1, 2A and 2C regions of the isolates were compared with the corresponding regions of echovirus type 9 Hill and Barty strains. Unlike Hill strain, Barty strain contained a C-terminal extension to the capsid protein VP1 with an RGD (argnine-glycine-aspartic acid) motif. To determine whether similar structural features were present in our isolates, their nucleotide sequences including the VP1 region were analyzed. All isolates exhibited the VP1 extension with the RGD motif. We concluded the Korean isolates in the year of 1993 as the echovirus type 9 Barty strain although the isolates showed 15-20% nucleotide sequence differences in the several genomic regions.
