Sensitivity performance and DNA damage of Pyramidon and Benzidine in blood strain testing
10.13618/j.issn.1001-5728.2018.02.015
- VernacularTitle:匹拉米洞与联苯胺在血痕预实验中的灵敏性及DNA损伤检测研究
- Author:
Qingshan WANG
1
;
Yingnan FAN
;
Shuhua NING
;
Yongmin YU
Author Information
1. 重庆市公安局物证鉴定中心
- Keywords:
forensic evidence;
preliminary test;
benzidine method;
pyramidon method
- From:
Chinese Journal of Forensic Medicine
2018;33(2):180-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective Blood trace and extraction is the important premising for forensic medicine appraisal reliability, this paper puts forward the blood trace and extraction method of using pyramidon. Methods Using pyramidon and benzidine to treat diluted fresh blood, and using Chelex-100 reagent treatment after the DNA in blood samples, and then applying the technology of PCR-STR and fluorescence detection test sample STR typing, observing the STR classification results and the detection sensitivity of DNA, comparing the different influence of two method on subsequent DNA - STR inspection. Results The pyramidon method can detect the minimum hemoglobin concentration is 25μg/mL,which was lower than 5μg/mL of the benzidine method. The results showed that the effective typing rate of the benzidine method was 18.09%, which was significantly lower than 96.67% of the method of the pyramidon method. The tail length of the detection group was 52.40±9.21, and the Oliva tail moment was 43.29±4.85%, and the tail intensity was 16.25±2.35, which was significantly higher than that of the pyramidon group (P < 0.01). Conclusions The sensitivity of the pyramidon method is moderate, and the influence of the following STR subtype and the nuclear DNA of the sample is significantly lower than that of benzidine, which can be used in the pre-experiment of blood stain in forensic medicine.
