Determination of aconitine in biological human sample by UPLC-MS/MS

VernacularTitle:超高效液相色谱-质谱联用法测定生物检材中乌头碱
Author: Yuan ZHANG ¹ ; Bo CHEN ; Meiling ZHANG ; Haiqin ZHAO ; Lin LIN ; Dan LIN ; Xianqin WANG
Author Information

1. 温州医科大学司法鉴定中心法医毒物实验室
Keywords: forensic toxicological analysis; UPLC-MS/MS; aconitine; biological sample
From: Chinese Journal of Forensic Medicine 2018;33(1):68-70
CountryChina
Language:Chinese
Abstract: Objective To develop a simple and fast UPLC-MS/MS method for determination of aconitine in biological human sample. Methods The biological human sample was treated by acetonitrile precipitation, and then analyzed on a UPLC C18(2.1mm×50mm, 1.7μm)column. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution, at a flow rate of 0.4 mL/min, at 40℃. UPLC-MS/MS was performed in ESI source with MRM mode for quantification. Results The linear range of the concentration were 0.5~500ng/mL in blood and 1~1000ng/mL for aconitine in blood for aconitine (r>0.995). The relative recoveries of aconitine were in the range of 91.3%~110.2%, and the extraction recoveries were in the range of 72.8%~83.5%. The RSDs of intra-days and inter-day were both less than 14%. Conclusion The method is a simple, fast and could be used for determination of aconitine in biological human sample.