Simultaneous determination of three drugs in saliva by UPLC-MS/MS
10.13618/j.issn.1001-5728.2018.01.016
- VernacularTitle:UPLC-MS/MS测定唾液中的3种毒品成分
- Author:
Meng ZHAO
1
;
Zhaohong WANG
;
Shuai LIU
;
Wenjing ZHANG
;
Hong LI
Author Information
1. 宁波大学医学院病理研究室
- Keywords:
forensic toxicological analysis;
UPLC-MS/MS;
methylamphetamine;
ketamine;
morphine;
saliva
- From:
Chinese Journal of Forensic Medicine
2018;33(1):65-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective A method of determination of methamphetamine, ketamine and morphine in saliva was established by super high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS). Methods The saliva samples after precipitation protein, in the ACQUITY UPLC BEH Phenyl(100mm×2.1mm,1.7μm) column chromatography, after the acetonitrile: 0.3% Formic acid solution gradient elution and through the ESI+ ionization mode MRM analysis and inspection. Results The linearity was estadlished in saliva between 4μg/L and 200μg/L, LOD was 0.2μg/L, LOQ was 4μg/L. the recovery was in the range of 87%-128%. Conclusion The method is simple, rapid and accurate for determination of methamphetamine, ketamine and morphine in saliva.
