Preparation of colloidal gold test strip against human SMCY antigen
10.13618/j.issn.1001-5728.2018.01.012
- VernacularTitle:抗人SMCY抗原胶体金试纸条的研制
- Author:
Zhiyuan AN
1
;
Huaigu ZHOU
;
Jianxia WANG
;
Xiaoyan FENG
;
Dong ZHAO
Author Information
1. 证据科学教育部重点实验室 中国政法大学
- Keywords:
forensic biology;
gender identification;
SMCY antigen;
colloidal gold
- From:
Chinese Journal of Forensic Medicine
2018;33(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid method for the detection of SMCY antigen. Methods To Use the technology of colloidal gold immunochromatography with double antibody sandwich assay, the gold labeled pad was coated with colloidal gold labeled SMCY rabbit polyclonal antibody, colloidal gold strip was made for detection of serum, which include 12 serum samples of pig, cattle, dog, chicken and mice and 50 serum samples of human. Results The colloidal gold test strips showed obvious specificity in human and common animal sera and could distinguish between male and female sera. Conclusion This method can be used to identify the serum of women and men, and has certain species specificity, which provides a direction for forensic science to rapidly identify gender of human samples.
