An exploratory study of effectiveness with ethylene oxide treatment for removing DNA contamination
10.13618/j.issn.1001-5728.2018.01.011
- VernacularTitle:环氧乙烷消杀DNA污染效果初探
- Author:
Haijun HAN
1
;
Yuhong ZHANG
;
Dongtao JIA
;
Min YANG
;
Hai YI
;
Haiyan QIN
;
Hao ZHANG
;
Qihui ZHU
Author Information
1. 南通市公安局物证鉴定所
- Keywords:
forensic biological evidence;
Ethylene Oxide;
DNA contamination;
STR profile
- From:
Chinese Journal of Forensic Medicine
2018;33(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective This exploratory study aimed to assess effectiveness with ethylene oxide treatment for removing DNA contamination. Methods 98 different spiked samples such as saliva, dander, skin cell, hair, blood and cartilage were conducted with ethylene oxide treatment. After extraction of samples, the dna was amplified and then the STR analysis was performed with 3130xl or 3500xl. Results A 6h EO treatment results showed that two saliva stains of 44 samples STR profile were detected; Just one hair of 54 samples treated with ethylene oxide was detected contaminating DNA with EO treatment for 8 hours. Conclusion This work suggested that it was more successful to reduce DNA contamination by using ethylene oxide treatment.
