Technical feasibility study on the application of circRNA in body fluid identification
10.13618/j.issn.1001-5728.2018.01.007
- VernacularTitle:circRNA应用于体液斑鉴定的技术可行性研究
- Author:
Hemiao ZHAO
1
;
Qingluan LIN
;
Jing CHEN
;
Wanshui LI
;
Chong WANG
Author Information
1. 广东省深圳市公安局福田分局
- Keywords:
forensic biological evidence;
circRNA;
body fluid identification
- From:
Chinese Journal of Forensic Medicine
2018;33(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using circRNA detection technology to explore the feasibility of the application of circRNA molecules in the identification of body fluids. Methods Prepare three kinds of body fluid samples: semen, saliva and vaginal secretions. Total RNA was extracted from Qiagen RNeasy Micro kit and digested by RNase R to obtain circRNA. Reverse transcription PCR amplification and agarose gel electrophoresis analysis were performed to detect target products. Results CircRNA can be detected in all prepared samples. These results showed that the circRNA was widely present in common body fluids of forensic medicine, and had some application value. Conclusion The detection for circRNA can be compatible with the existing DNA detection technology, and its tissue specificity can be used as a new marker for identification of body fluid and has important research value.
