Development of a 30 Y-STR Loci with middle or low mutations multiplex PCR system
10.13618/j.issn.1001-5728.2018.01.003
- VernacularTitle:30个中低突变Y-STR基因座复合扩增体系的建立
- Author:
Weiwei WU
1
;
Honglei HAO
;
Huaifeng WANG
;
Bing LIU
;
Xinglin MEI
;
Xiang ZHOU
;
Yanjia SU
;
Wenyan REN
;
Yanfang FU
;
Xiaoting ZHENG
;
Dejian LV
Author Information
1. 浙江省公安物证鉴定中心 浙江省刑事科学技术应用研究重点实验室
- Keywords:
forensic biological science;
Y-chromosomal;
STR;
middle or low mutation rate;
labelled with fluorescence;
multiplex PCR
- From:
Chinese Journal of Forensic Medicine
2018;33(1):11-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To deveplope construct and validate a novel multiplex PCR system comprised of 30 Y-STR markers only with low and moderate mutation rates. Methods 30 Y-STRs characterized by low/moderate mutation rate and middle/high polymorphic was amplified simultaneously in a multiplex PCR system using the six color labeling fluorescence. PCR product was analyzed in a ABI 3500XL Genetic Analyzer. The accuracy, specifity, sensitivity and stability of the system and its validation on the mixtures were evaluated. Results The validation studies demonstrated that the system is a stable, accurate, and sensitive multiplex PCR system. The sensitivity was 0.0625ng DNA. Y-STR could be detection in a male/female DNA mixture ratio of 1:4. Conclusion The primary study demonstrates that this multiplex PCR system is effective and reliable for forensic routine DNA analysis. It will be very helpful for constructing Chinese forensic Y-STR database and population genetic research.
