Establishment and application of miRNA detection method for forensic body fluid identification
10.13618/j.issn.1001-5728.2018.01.002
- VernacularTitle:体液法医学鉴定miRNA检测方法的建立与应用
- Author:
Ranran LI
1
;
Xing MA
;
Yalin LI
;
Yingying SUN
;
Anquan JI
;
Hui TANG
;
Caixia LI
;
Qifan SUN
Author Information
1. 山西医科大学,山西 太原 030001
- Keywords:
forensic genetics;
body fluid identification;
real-time PCR;
miRNA
- From:
Chinese Journal of Forensic Medicine
2018;33(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a SYBR Green real-time PCR detection method with tissue-specific miRNAs and explore a novel approach for forensic body fluid identification. Methods The frequently reported 6 standard miRNAs were synthesized to establish a SYBR Green method, and verify with body fluid. The relative expression data for the 6 miRNAs were obtained using SYBR Green real-time PCR method in peripheral blood, menstrual blood, saliva and semen. Results The assays showed that miRNA205 permitted the unequivocal identification among different fluids. miRNA451 and miRNA144 could be used to distinguish blood from non-blood. Menstrual blood or peripheral blood could be identified through miRNA214. miRNA888 and miRNA891 was highly expressed in semen. Conclusion The results of this study indicate that miRNA SYBR Green profiling may provide a feasible and effective approach to body fluid identification for forensic casework.
