An uncertainty assessment method for determination of iodine in urine by As3+-Ce4+ catalytic spectrophotometry
10.3760/cma.j.issn.2095-4255.2018.11.014
- VernacularTitle:尿中碘砷铈催化分光光度法测量的不确定度评估
- Author:
Yanguo JIANG
1
;
Peng LI
Author Information
1. 辽宁省丹东市疾病预防控制中心检测检验所
- Keywords:
Urine;
Iodine;
Spectrophotometry;
Uncertainty;
Assessment
- From:
Chinese Journal of Endemiology
2018;37(11):912-916
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up an uncertainty assessment method for urinary iodine measurement by As3+-Ce4+ catalytic spectrophotometry,in order to assess the accuracy of this method and find out the major influence factors.Methods A mathematical model was established based on the determination method to evaluate the uncertainty component of various factors such as potassium iodate benchmark,standard curve calibration,glass equipment,balance weighing,temperature control instrument,etc.,and the uncertainty of the test results of urinary iodine standard substances was analyzed.Results The iodine content of the urinary iodine standard material GBW09108i was (72.6 ± 3.7) μg/L,and the relative expansion uncertainty was 5.10％,within the tolerance range allowed by the reference material certificate (10.0％).Among them,the calibration of the standard curve,the uncertainty of the volume of the glass equipment and related consumables had a great influence on the extended uncertainty.Conclusion The method can be used to assess the uncertainty for determination of iodine in urine by As3+-Ce4+ catalytic spectrophotometry.
