Molecular identification of main vector fleas in Qinghai plague foci by DNA barcoding
10.3760/cma.j.issn.2095-4255.2018.07.004
- VernacularTitle:基于DNA条形码技术对青海鼠疫疫源地内主要媒介蚤的分子鉴定研究
- Author:
Ying MA
1
;
Hailong LI
;
Jian HE
;
Yanmei ZHAO
;
Hanqing YANG
;
Liang LU
;
Qiyong LIU
Author Information
1. 青海省地方病预防控制所
- Keywords:
Siphonaptera;
Cytochrome c oxidase subunit Ⅰ;
DNA barcoding
- From:
Chinese Journal of Endemiology
2018;37(7):532-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective To make up the limitations of traditional morphological classification methods,we identified vector fleas by DNA barcoding in Qinghai Province.Methods The mt DNA cytochrome c oxidase subunit Ⅰ (CO Ⅰ) gene was amplified by PCR from 36 muscle tissues of fleas in 3 states,2 cities and 5 counties of Qinghai Province,and the obtained CO Ⅰ gene fragments were sequenced and aligned.The intra-and inter-species genetic distances were calculated with Mega 6 software using K2-P model and a phylogenetic tree was constructed with neighbor-joining (NJ) method.Results Totally 36 CO Ⅰ gene sequences of 2 superfamilies,4 genera and 6 kinds of vector fleas were measured,the average genetic distance was 0.119,and the intraspecific distance was 0.002-0.027,the interspecific distance was 0.039-0.207,and the interspecific genetic distance was significantly greater than the intraspecific genetic distance.NJ tree showed the same species had formed a single line with high support rate and interspecific branch was clear.Conclusion DNA barcoding is suitable for identification of vector fleas in Qinghai Province,may make up the limitations of traditional morphological classification methods.
