Direct sampling introduction and atomic fluorescence spectrometry for determination of selenium content in mice liver and urine
10.3760/cma.j.issn.2095-4255.2018.02.015
- VernacularTitle:直接进样原子荧光法对小鼠肝脏和尿硒含量测定
- Author:
Jinli CHEN
1
;
Li FENG
;
Cang QI
;
Yongfang LI
;
Da WANG
;
Bing LI
;
Guifan SUN
Author Information
1. 中国医科大学公共卫生学院环境与慢病研究中心
- Keywords:
Direct introduction;
Atomic fluorescence spectrometry;
Liver;
Urine;
Selenium
- From:
Chinese Journal of Endemiology
2018;37(2):149-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through determination of selenium content in liver and urine of selenium-induced mice, direct sampling atomic fluorescence spectrometry was established to provide a more accurate and convenient determination method for detection of selenium-related biological samples. Methods Selenium in the sample was released by the electrically heated quartz tube,the selenium in the atomic state was captured by the quartz tube,and the selenium released by the heating quartz tube was carried by the argon-hydrogen mixed gas into the argon-argon flame atomic fluorescence detector for determination; standard curve was established based on selenium content and fluorescence area, and then the content of selenium in the sample was calculated. Results The detection limit of selenium in samples by direct sampling atomic fluorescence spectrometry was 0.28 μg/kg, the correlation coefficient of standard curve was 0.999 3, and the relative standard deviation range was 1.82% - 4.19%. The adding standard recovery of the liver in mice was 87.30%- 100.20%; meanwhile the adding standard recovery of the urine in mice was 93.10% - 96.60%. Conclusions Direct sampling atomic fluorescence method is simple and easy to operate, accuracy and precision are better, the linear range is wide. The samples need not be processed by complex pretreatment,such as acid,etc.,elements loss is avoided and efficiency of detection is improved.The method can be used in a variety of samples for rapid detection of trace selenium.
